the flame champion lived with sana for only a few years before he died. this would indicate he couldnt survive without his rune since he had it for a while, or it could be that the way he forced the TFR from him{sindarian technique}, shortened his life spanthe first ex is former FC , he lived with sanar after have been carried a TFR and he said 50year fire-ceased from harmonia 's long enough for him to be with his love so that mean after split up with the rune , he aged normally and died.
the second ex is hmm I dunt remember his name Is it Clay or
Graham or smthing? the last enemy in suik IV , as he was once holding True rune of punishment and then to escape the curse , he cut off his right arms and he's still living for so long just to died in suik IV(lol).
graham cray only had the rune for a year or so{not eactly sure} but not long enough for his aging to be affected, his saving grace was being able to seperate from the rune of punishment with his life intact
Ted didn't age because the fogship captain granted him immortality while ted was on the fog ship, so ted did not age on the fogship, why he looks older in 4 and younger in 1 is a mystery,probably an artist thingand the third exam 'd be Ted he lost his true rune twice , the first time by giving it to the fogship captain , through the story didn't describe how long had he given it to the Captain but I believe it'd be more than 10years until he found Hero IV and once again he gave it to Tir and he still live long enough to died just a bit before the war in suik I ends.